Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.
Identifieur interne : 000A81 ( Main/Exploration ); précédent : 000A80; suivant : 000A82Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.
Auteurs : B. Van Aken [Belgique] ; M D Cameron ; J D Stahl ; A. Plumat ; H. Naveau ; S D Aust ; S N AgathosSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2000.
Descripteurs français
- KwdFr :
- Dépollution biologique de l'environnement (MeSH), Dérivés de l'aniline (métabolisme), Glutathion (métabolisme), Manganèse (métabolisme), Marqueurs de spin (MeSH), Oxydoréduction (MeSH), Peroxidases (métabolisme), Phanerochaete (enzymologie), Radicaux libres (MeSH), Spectroscopie de résonance de spin électronique (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Aniline Compounds, Glutathione, Manganese, Peroxidases.
- enzymology : Phanerochaete.
- Biodegradation, Environmental, Electron Spin Resonance Spectroscopy, Free Radicals, Oxidation-Reduction, Spin Labels.
Abstract
Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.
DOI: 10.1007/s002530000436
PubMed: 11131391
Affiliations:
- Belgique
- Province du Brabant wallon, Région wallonne
- Louvain-la-Neuve
- Université catholique de Louvain
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.</title><author><name sortKey="Van Aken, B" sort="Van Aken, B" uniqKey="Van Aken B" first="B" last="Van Aken">B. Van Aken</name><affiliation wicri:level="4"><nlm:affiliation>Unit of Bioengineering, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Unit of Bioengineering, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Cameron, M D" sort="Cameron, M D" uniqKey="Cameron M" first="M D" last="Cameron">M D Cameron</name></author><author><name sortKey="Stahl, J D" sort="Stahl, J D" uniqKey="Stahl J" first="J D" last="Stahl">J D Stahl</name></author><author><name sortKey="Plumat, A" sort="Plumat, A" uniqKey="Plumat A" first="A" last="Plumat">A. Plumat</name></author><author><name sortKey="Naveau, H" sort="Naveau, H" uniqKey="Naveau H" first="H" last="Naveau">H. Naveau</name></author><author><name sortKey="Aust, S D" sort="Aust, S D" uniqKey="Aust S" first="S D" last="Aust">S D Aust</name></author><author><name sortKey="Agathos, S N" sort="Agathos, S N" uniqKey="Agathos S" first="S N" last="Agathos">S N Agathos</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:11131391</idno><idno type="pmid">11131391</idno><idno type="doi">10.1007/s002530000436</idno><idno type="wicri:Area/Main/Corpus">000A74</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A74</idno><idno type="wicri:Area/Main/Curation">000A74</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A74</idno><idno type="wicri:Area/Main/Exploration">000A74</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.</title><author><name sortKey="Van Aken, B" sort="Van Aken, B" uniqKey="Van Aken B" first="B" last="Van Aken">B. Van Aken</name><affiliation wicri:level="4"><nlm:affiliation>Unit of Bioengineering, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Unit of Bioengineering, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Cameron, M D" sort="Cameron, M D" uniqKey="Cameron M" first="M D" last="Cameron">M D Cameron</name></author><author><name sortKey="Stahl, J D" sort="Stahl, J D" uniqKey="Stahl J" first="J D" last="Stahl">J D Stahl</name></author><author><name sortKey="Plumat, A" sort="Plumat, A" uniqKey="Plumat A" first="A" last="Plumat">A. Plumat</name></author><author><name sortKey="Naveau, H" sort="Naveau, H" uniqKey="Naveau H" first="H" last="Naveau">H. Naveau</name></author><author><name sortKey="Aust, S D" sort="Aust, S D" uniqKey="Aust S" first="S D" last="Aust">S D Aust</name></author><author><name sortKey="Agathos, S N" sort="Agathos, S N" uniqKey="Agathos S" first="S N" last="Agathos">S N Agathos</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="ISSN">0175-7598</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aniline Compounds (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Electron Spin Resonance Spectroscopy (MeSH)</term><term>Free Radicals (MeSH)</term><term>Glutathione (metabolism)</term><term>Manganese (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Spin Labels (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Dépollution biologique de l'environnement (MeSH)</term><term>Dérivés de l'aniline (métabolisme)</term><term>Glutathion (métabolisme)</term><term>Manganèse (métabolisme)</term><term>Marqueurs de spin (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Radicaux libres (MeSH)</term><term>Spectroscopie de résonance de spin électronique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aniline Compounds</term><term>Glutathione</term><term>Manganese</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dérivés de l'aniline</term><term>Glutathion</term><term>Manganèse</term><term>Peroxidases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Electron Spin Resonance Spectroscopy</term><term>Free Radicals</term><term>Oxidation-Reduction</term><term>Spin Labels</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Marqueurs de spin</term><term>Oxydoréduction</term><term>Radicaux libres</term><term>Spectroscopie de résonance de spin électronique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11131391</PMID><DateCompleted><Year>2001</Year><Month>02</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0175-7598</ISSN><JournalIssue CitedMedium="Print"><Volume>54</Volume><Issue>5</Issue><PubDate><Year>2000</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>659-64</MedlinePgn></Pagination><Abstract><AbstractText>Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Van Aken</LastName><ForeName>B</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Unit of Bioengineering, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cameron</LastName><ForeName>M D</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Stahl</LastName><ForeName>J D</ForeName><Initials>JD</Initials></Author><Author ValidYN="Y"><LastName>Plumat</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Naveau</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Aust</LastName><ForeName>S D</ForeName><Initials>SD</Initials></Author><Author ValidYN="Y"><LastName>Agathos</LastName><ForeName>S N</ForeName><Initials>SN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000814">Aniline Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005609">Free Radicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013113">Spin Labels</NameOfSubstance></Chemical><Chemical><RegistryNumber>189OOM840S</RegistryNumber><NameOfSubstance UI="C035208">2-amino-4,6-dinitrotoluene</NameOfSubstance></Chemical><Chemical><RegistryNumber>42Z2K6ZL8P</RegistryNumber><NameOfSubstance UI="D008345">Manganese</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000814" MajorTopicYN="N">Aniline Compounds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004578" MajorTopicYN="N">Electron Spin Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005609" MajorTopicYN="N">Free Radicals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008345" MajorTopicYN="N">Manganese</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013113" MajorTopicYN="N">Spin Labels</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>12</Month><Day>29</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>3</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>12</Month><Day>29</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11131391</ArticleId><ArticleId IdType="doi">10.1007/s002530000436</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province du Brabant wallon</li><li>Région wallonne</li></region><settlement><li>Louvain-la-Neuve</li></settlement><orgName><li>Université catholique de Louvain</li></orgName></list><tree><noCountry><name sortKey="Agathos, S N" sort="Agathos, S N" uniqKey="Agathos S" first="S N" last="Agathos">S N Agathos</name><name sortKey="Aust, S D" sort="Aust, S D" uniqKey="Aust S" first="S D" last="Aust">S D Aust</name><name sortKey="Cameron, M D" sort="Cameron, M D" uniqKey="Cameron M" first="M D" last="Cameron">M D Cameron</name><name sortKey="Naveau, H" sort="Naveau, H" uniqKey="Naveau H" first="H" last="Naveau">H. Naveau</name><name sortKey="Plumat, A" sort="Plumat, A" uniqKey="Plumat A" first="A" last="Plumat">A. Plumat</name><name sortKey="Stahl, J D" sort="Stahl, J D" uniqKey="Stahl J" first="J D" last="Stahl">J D Stahl</name></noCountry><country name="Belgique"><region name="Région wallonne"><name sortKey="Van Aken, B" sort="Van Aken, B" uniqKey="Van Aken B" first="B" last="Van Aken">B. Van Aken</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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